Hello Anne,
Thank you for posting question on Microsoft Windows Forum!
Based on the issue description. Well! One of the possible causes for this is Default Printer Change. If your default printer was changed (e.g., to a new network printer or a PDF driver) that has "Portrait" as its hardcoded default, Excel may re-evaluate the spreadsheets to match that driver’s capabilities. Also, a recent update to Microsoft 365 or a pushed GPO setting regarding printing/Office templates could have reset the default global Book.xltx or Sheet.xltx templates.
Another point worth mentioning here is the "Select All Sheets" Trap. If these files were edited by a user who right-clicked a tab and chose Select All Sheets, any change made to the Page Layout on one tab would have instantly applied to every sheet in that specific file.
To prevent this from recurring, check if your Default Printer was recently changed. If you use a "Print to PDF" driver as your default, ensure its internal settings are set to Landscape, or switch your default printer to one with more flexible driver settings.
Please note: Make a full backup of the folder (copy the entire folder to a different location or external drive).
If the files are stored/synced to OneDrive or SharePoint, you may be able to restore them to a previous point in time (version history or library/account-level restore). On the other hand, OneDrive/SharePoint offers version history per file, SharePoint/OneDrive for Business also has library/account-level “restore” features that roll many files back to a chosen date. This is ideal if the orientation change happened recently and you want the exact prior state.
Hope the above information is helpful! If it is. It is appreciated to consider clicking "Accept Answer".